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TLC Products

We offer a complete line of products for thin layer chromatography (TLC), high performance TLC (HPTLC) and preparative TLC, including plates, reagents and accessories.

G

Glass Plates

P

Polygram® Polyester Sheets

A

Alugram® Aluminium Sheets

AX

Alugram® Xtra Aluminium Sheets

Phases for TLC

Supports Phase Description
Standard silica
G ADAMANT silica 60, improved binder system, optimized particle size distribution
G, P,A,AX SIL G ALUGRAM® Xtra SIL G silica 60, standard grade, particle size 5–17 µm
ALUGRAM® Xtra: as above, on aluminium, outstanding wettability, improved cutting properties
G DURASIL silica 60, special binder system
P SIL N-HR high purity silica 60, special binder system, higher gypsum content
G,AX SILGUR silica 60 with kieselguhr concentrating zone
Unmodified silica for HPTLC
G,AX Nano-SILGUR nano silica 60 with kieselguhr concentrating zone
G Nano-ADAMANT nano silica 60, optimized binder system and particle size distribution
G,A,AX Nano-SIL nano silica 60, standard grade, particle size 2–10 µm
G Nano-DURASIL nano silica 60, special binder system
G AMD SIL nano silica 60, extremely thin layer for AMD procedure
Modified silica for HPTLC
G Nano-SIL C18-50 / C18-100 nano silica with partial or complete C18 modification
G,A RP-18 W nano silica with partial octadecyl modification, wettable with water
G,A RP-2 silanized silica = dimethyl-modified silica 60
G,A Nano-SIL CN cyano-modified nano silica
G,A Nano-SIL NH2 amino-modified nano silica
G,A Nano-SIL Diol diol-modified nano silica
Aluminium oxide
G,P,A Alox-25 / Alox N aluminium oxide
Cellulose, unmodified and modified
G,P,A CEL 300 native fibrous cellulose MN 300
G,P CEL 400 microcrystalline cellulose MN 400 (AVICEL)
P CEL 300 DEAE dimethylaminoethyl-modified cellulose ion exchanger
P CEL 300 DEAE/HR mixed layer of cellulose ion exchanger and high purity cellulose
P CEL 300 PEI polyethyleneimine-impregnated cellulose ion exchanger
G,P CEL 300 AC acetylated cellulose MN 300
Layers for special separations
P Polyamide 6 perlon = ε-aminopolycaprolactame
G CHIRALPLATE RP-silica with Cu2+ ions and chiral reagent, for enantiomer separation
G SIL G-25 HR high purity silica 60 with gypsum, recommended for aflatoxin analysis
G SIL G-25 Tenside silica G with ammonium sulfate for separation of surfactants
G GUR N Kieselguhr
G Nano-SIL PAH nano silica with special impregnation for PAH analysis
P IONEX-25 SA-Na mixed layer of strongly acidic cation exchanger and silica
P IONEX-25 SB-AC mixed layer of strongly basic anion exchanger and silica
G Alox/CEL-AC-Mix mixed layer of aluminium oxide and acetylated cellulose
G SILCEL-Mix mixed layer of cellulose and silica
G GURSIL-Mix mixed layer of kieselguhr and silica

TLC/ HPTLC Plates

ALUGRAM® Xtra SIL G unmodified standard silica layers on aluminium

ALUGRAMXTRA_SILG4x8

  • silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 ml/g, particle size 5 – 17 µm; standard grade
  • outstanding wettability for precise colorization results, even with 100 % aqueous eluents
  • excellent separation efficiency and reproducibility from lot to lot easy and reliable cutting due to an optimized binder system, no flaking of silica
  • Binder: highly polymeric product, which is stable in almost all organic solvents and resistant towards aggressive visualisation reagents; also completely stable in purely aqueous eluents

RP-18 W/UV254 octadecyl-modified nano silica layers

ALUGRAMXtra20x20

  • base material: silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 ml/g, mean particle size 9 μm, pH stability 2 – 10 indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm); UV-absorbing substances apppear as dark-blue to black spots on a light-blue background
  • partial octadecyl (C18) modification, wettable with water, carbon content 14 %
  • order of polarity: silica > DIOL > NH2 > CN > RP-2 > C 18-50 > RP-18 W > C 18-100
  • normal phase or reversed phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water the relative polarity of the eluent determines the polarity of the layer
  • recommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)

CHIRALPLATE special layer for TLC enantiomer separation

  • reversed phase nano silica impregnated with Cu2+ ions and a chiral selector (a proline derivative, DP 31 43 726 and EP 0 143 147)
  • separation based on ligand exchange, i. e. formation of ternary mixed-ligand complexes with the Cu(II) ions; differences in the stability of the diastereomeric complexes cause chromatographic separation
  • recommended application: enantiomer separation of amino acids, N-methylamino acids, N-formylamino acids, α-alkylamino acids, thiazolidine derivatives, dipeptides, lactones, α-hydroxycarboxylic acids
For details on other TLC Plases, plese click here

HPTLC method development kits

for selection of the optimum HPTLC plate for a given separation

Kit 1 Glass Plates 3 plates 10 x 10 cm (scored to 5 x 10 cm) each of Nano-SIL C18-100/UV254, RP-18 W/UV254, RP-2/UV254, Nano-SIL CN/UV, Nano-SIL NH2/UV, Nano-SIL DIOL/UV
Kit 2 ALUGRAM® Aluminium Sheets 5 sheets 4 x 8 cm each of RP-18 W/UV254, RP-2/UV254, Nano-SIL CN/UV, Nano-SIL NH2/UV, Nano-SIL DIOL/UV

TLC Micro Sets

TLC micro-set A for beginners

This kit contains all chemicals and accessories for the following separations:

  • separation of the fat-soluble (lipophilic) dye mix-ture 1: butter yellow, indophenol, sudan blue II, sudan red G
  • separation of a mixture of anthraquinone dyes (test dye mixture 2): blue 1, blue 3, green, green blue, red, violet 1, violet 2
  • separation of a mixture of food dyes (test dye mixture 3): brilliant black BN (E151), fast red E, erythrosine (E127), yellow orange S (sunset yellow CFC, E110), naphthol red S, ponceau 4 R (E124), tartrazine (E102)
  • separation of dyes from felt tip pens

TLC micro-set F 1

This kit contains all chemicals required for the separation of

  • amino acids (test mixture, consisting of alanine, arginine, tryptophan and valine)
  • amino acids in urine
  • the heavy metal cations copper(II), manganese(II),and nickel(II)
TLC Micro Sets

TLC micro-set

TLC micro-set F 2

This kit contains all chemicals required

  • for the analysis of edible fats
  • as well as for analysis of fats and cholesterol in blood

TLC micro-set F 3

This kit contains all chemicals required

  • for the separation of analgetics (pain relievers)
  • and for drug analysis as shown for cinchona bark

TLC wine set

This kit contains all chemicals and equipment required for determination of malic, lactic, and tartaric acid in wine (evaluation of the conversion of malic to lactic acid, andfermentation)

Visualisation Reagents

Visualisation reagents

  • a small selection of frequently used spray reagents for postchromatographic detection reactions in TLC suited for spraying or dipping TLC plates
  • a detailed description of many more detection procedures for TLC is available on request

Adsorbents for TLC

Silica

pore size 60 Å, pore volume 0.75 ml/g, specific surface (BET) ~ 500 m2/g, pH of a 10 % aqueous suspension 7.0

Silica G

standard grade, particle size 2 – 20 μm, Fe < 0.02 %, Cl < 0.02 %, 13 % gypsum as binder, supplied with or without fluorescence indicator UV254

Silica N

standard grade, particle size 2 – 20 μm, Fe < 0.02 %, Cl < 0.02 %, no binder, supplied with or without fluorescence indicator UV254

Silica G-HR

high purity grade, particle size 3 – 20 μm, Fe < 0.002 %, Cl < 0.008 %, gypsum as binder, supplied without fluorescence indicator

Silica P

preparative grade, particle size 5 – 50 μm, Fe < 0.02 %, Cl < 0.02 %, organic binder, supplied with fluorescence indicator UV254

Silica P with gypsum

preparative grade, particle size 5 – 50 μm, Fe < 0.02 %, Cl < 0.02 %, gypsum as binder, supplied with fluorescence indicator UV254

Aluminium oxide adsorbents for TLC

pore size 60 Å, specific surface (BET) ~ 200 m2/g

  • Aluminium oxide G ~ 10 % gypsum as binder, supplied with or without fluorescence indicator
  • Aluminium oxide N no binder, supplied without fluorescence indicator

Polyamide adsorbents for TLC

  • Polyamide 6 = nylon 6 = perlon = ε-aminopolycaprolactame

Cellulose MN 301 native fibrous cellulose

  • fibre length (95%) 2 – 20 μm, average degree of polymerisation 400 – 500, specific surface acc. to Blaine 15000 cm2/g
  • Cellulose MN 301: native fibrous cellulose, standard grade
    ≤ 20 ppm Fe, 6 ppm Cu, 7 ppm P, CH2Cl2 extract ≤ 0.25%, residue on ignition at 850 °C ≤ 1500 ppm
  • Cellulose MN 301 HR: fibrous cellulose, high purity grade, acid-washed and defatted
    ≤ 2 ppm Fe, 1 ppm Cu, CH2Cl2 extract ≤ 0.025%, residue on ignition at 850 °C ≤ 200 ppm recommended for quantitative investigations, e. g. for separation of carbohydrates with subsequent IR spectroscopy or separation of phosphoric acids, phosphates etc.
  • Cellulose MN 301 A: special grade for the 32P postlabelling procedure
    ≤ 20 ppm Fe, ≤ 6 ppm Cu, ≤ 7 ppm P, CH2Cl2 extract ≤ 0.01%, residue on ignition at 850 °C ≤ 500 ppm free of lactobacili contaminations; not impregnated with PEI, but designed for impregnation and coating by the user

Fluorescent indicators

UV indicators with efficient radiation for short-wave as well as long-wave UV ranges
UV254: manganese activated zinc silicate with absorption maximum at 254 nm; green fl uorescence; relatively susceptible towards acids; thus its fluorescence can be completely quenched by acidic solvents
UV366: inorganic fluorescent pigment with absorption maximum at 366 nm; blue fluorescence

Accessories for TLC

TLC UV Cabinet (RASUVC1)

UV Cabinet with Three Light Source

  • Long Wave 365nm
  • Short Wave 254nm
  • Visible Light
TLC UV Cabinet

TLC UV Cabinet

TLC UV Cabinet (RASUVC2)

UV Cabinet with Three Light Source

  • Long Wave 365nm
  • Short Wave 254nm
  • Visible Light

Special Features

  • Flicker Less Illumination
  • Total Dark Room
  • Imported Lamp Filters for TRUE UV
  • Micro Controller based Timer
TLC UV Cabinet

TLC UV Cabinet

UV Lamps for UV Cabinets

Short and Long wave

Short and Long wave

TLC UV Lamp Portable

  • Portable UV Lamp, Long & Short Wave (365nm & 254nm)
UV-Lamps portable

UV-Lamps portable

TLC Developing Chamber

  • 20 x 20cm
  • 10 x 20cm
  • 10 x 10cm
TLC Developing Tank

TLC Developing Tank

Syringes for TLC

  • Syringes with TEFLON® -coated needle tip for reproducible sample spottin
Syringes for TLC

Syringes for TLC

Cutting tool for TLC

  • TLC Scissor
TCutting tool for TLC

Cutting tool for TLC

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