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MN HPLC Columns

NUCLEOSIL

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure

  • Wide acceptance as routine packings for very different fields of chromatography.
  • One of the first spherical silicas used in HPLC.
  • An absolutely reliable choice in HPLC.
  • The largest variety of modified HPLC silicas available on the market.

Summary Of Available NUCLEOSIL Phases

Phase Modification % C pH Range Particle Sizes [µm] USP Classification
RP phases
NUCLEOSIL® C18 medium density octadecyl, endcapped 15 2-8 3,5,7,10 µm L1
NUCLEOSIL® C18 AB crosslinkedoctadecyl, endcapped, high steric selectivity 25 1-9 5 µm L1
NUCLEOSIL® C18 HD high density octadecyl, endcapped 20 2-9 3,5 µm L1
NUCLEOSIL® SiOH Unmodified – 2-8 5 µm L3
NUCLEOSIL® C8 ec medium density octyl, endcapped 8.5 2-8 5 µm L7
NUCLEOSIL® C8 standard octyl, not endcapped 8.5 2-8 3,5,7,10 µm L7
NUCLEOSIL® C8 HD high density octyl, endcapped 13 2-8 5 µm L7
NUCLEOSIL® NH2 aminopropyl -(CH2)3-NH2 3.5 2-8 5,10 µm L8
NUCLEOSIL® SA benzenesulfonic acid, strongly acidic cation exchanger (SCX) 6.5 2-8 5,10 µm L9
NUCLEOSIL® CN cyanopropyl (nitrile) 5 2-8 5,7,10 µm L10
NUCLEOSIL® Phenyl standard phenyl, not endcapped 8 2-8 5,7 µm L11
NUCLEOSIL® SB quaternary ammonium groups, strongly basic anion exchanger (SAX) 10 2-8 5,10 µm L14
NUCLEOSIL® C2 dimethyl 3.5 2-8 7 µm L16
NUCLEOSIL® OH diol (dihydroxypropyl) 5 2-8 5,7 µm L20
NUCLEOSIL® C4 medium density butyl, endcapped 2 2-8 5 µm L26
NUCLEOSIL® C18 Nautilus hydrophilic octadecyl, embedded polar group, endcapped 16 2-8 3,5 µm L60
NUCLEOSIL® Protect I special RP phase with protective polar group, endcapped 11 2-8 5 µm –

NUCLEODUR®

NUCLEODUR® ® is a fully synthetical type B silica (silica of 3rd generation) offeringhighly advanced physical properties:

  • Totally spherical particle shape
  • Outstanding surface microstructure
  • High pressure stability
  • Low metal content

Summary Of Available NUCLEODUR® Phases

Phase Modification % C pH Range Particle Sizes [µm] USP Classification
NUCLEODUR®  C18 Gravity Octadecyl, high density coating, multi-endcapping 18 1–11 1.8, 3, 5 L1
NUCLEODUR®  C18 Gravity SB Monomeric octadecyl modification, extensive endcapping; 13 1-9 1.8, 3, 5 L1
NUCLEODUR®  C18 HTec Octadecyl, high density coating, high capacity, multi-endcapping 18 1-11 1.8, 3, 5, 7, 10 L1
NUCLEODUR®  C18 Isis Octadecyl, specially cross-linked modification, endcapped 20 1-10 1.8, 3, 5 L1
NUCLEODUR®  C18 Pyramid Octadecyl with polar endcapping 14 1-9 1.8, 3, 5 L1
NUCLEODUR®  PolarTec Octadecyl with embedded polar group 17 1-9 3,5 L1 L60
NUCLEODUR®  C18 ec Octadecyl, medium density, endcapping 17.5 1–9 3, 5 and larger L1
NUCLEODUR®  SiOH Unmodified NUCLEODUR® ® – 3, 5 and larger L3
NUCLEODUR®  C8 Gravity Octyl, high density coating, multi-endcapping 11 1-11 1.8, 5 L7
NUCLEODUR®  C8 ec Octyl, medium density, endcapping 10.5 1-9 3,5 L7
NUCLEODUR®  NH2/NH2-RP Aminopropyl for NP and RP separations 2.5 2–8 3, 5 L8
NUCLEODUR®  CN/CN-RP Cyano (nitrile) for NP and RP separations 7 1-8 3,5 L10
NUCLEODUR® π² Hydrophobic phase with alternative selectivity compared to classical C18 modifications 17 3-10 5 L11
NUCLEODUR®  PFP Pentafluorophenyl-propyl with multi-endcapping 8 1-9 3,5 L43
NUCLEODUR®  Sphinx RP Bifunctional, balanced ratio of propylphenyl and octadecyl, endcapping 15 1-10 1.8, 3, 5 L1 L11
NUCLEODUR®  HILIC Ammonium – sulphonic acid 7 2–8.5 1.8, 3, 5

NUCLEOSHELL®

  • Solid core of silicon dioxide,homogeneous shell of porous silica.
  • Highest efficiency compared to traditional totally porous materials.
  • Pore size 90 Ã…; particle size 2.7 µm (core 1.7 µm); specific surface 130 m2/g.
  • Lower back pressure also allows use on conventional LC systems.
  • Pressure stability up to 600 bar.
Phase Modification % C pH Range Particle Sizes [µm] USP Classification
NUCLEOSHELL®  RP 18 Octadecyl modification, multi-endcapped 7.8 for 2.7µm 6.1  for 5µm 1–11.0 2.7 µm, 5 µm L1
NUCLEOSHELL®  RP 18plus polar octadecyl modification, multi-endcapped 5.7 for 2.7µm 4.4 for 5µm 2-9 2.7 µm, 5 µm L1
NUCLEOSHELL®  Bluebird RP18 Special octadecyl core-shell phase with hydrophilic endcapping 5 1-8 2.7 µm (core 1.7 µm) L1
NUCLEOSHELL®  Phenyl-Hexyl phenyl-hexyl modification, multi-endcapped, 4.5 % C 4.5 1-10 2.7 µm L11
NUCLEOSHELL®  HILIC Ammonium – sulfonic acid modification 1.3 2–8.5 2.7 µm
NUCLEOSHELL®  PFP Pentafluorophenyl modification,  multi-endcapped ~ 3 1.0–9.0 2.7 µm L43

Columns for Special Applications

Separation / Mechanism Recommended Column Specification Of The Phase USP Classification
Environmental analysis
anion exchangechromatography of inorganic anions NUCLEOGEL®Anion I strongly basic polymer-based anion exchanger
NUCLEOSIL®Anion II strongly basic silica-based anion exchanger
RP chromatography of PAHs NUCLEODUR® ®C18PAH, 3µm NUCLEODUR® ®polymer-coated with C18 groups L1
NUCLEOSIL®100-5 C18PAH NUCLEOSIL®100 polymer-coated with C18groups L1
Enantiomer separation
based on formation of inclusion complexes NUCLEODEX α-PM, β-PM, γ-PM and β-OH silica-based permethylated and underivatisedcyclodextrin phases L45
based on polar and π-π interactions NUCLEOCEL DELTA silica-based modified cellulose phases L40
NUCLEOCEL ALPHA silica-based modified amylosephases L51
based on ligand exchange NUCLEOSIL®CHIRAL-1 covalently bonded amino acid – Cu(II) complexes L32
based on charge-transfer-, dipole-dipole interactions and others NUCLEOSIL® CHIRAL-2 NUCLEOSIL®CHIRAL-3 silica-based brush type phases L36
based on enantioselective bindingto chiral protein surface structures RESOLVOSIL BSA-7 silica-based protein phase (BSA)
Biological macromolecules
anion exchangechromatography of proteins and peptide NUCLEOSIL®4000-7 PEI silica-based polymeric poly ethyleneimine network
anion exchange chromatography of oligonucleotides and nucleic acids NUCLEOGEN®DEAE silica-based DEAE anion exchanger
anion exchange chromatography of peptides, large proteins and oligonucleotides NUCLEOGEL®SAX polymer-based strongly basic anion exchanger L23
cation exchange chromatographyof proteins, peptides and carbohydrates NUCLEOGEL®SCX polymer-based strong cation exchanger L22
reversed phase chromatography of proteins, peptides and oligonucleotides NUCLEOSIL®MPN monomerically bonded alkyl chains on silica L1L26
NUCLEOSIL®PPN polymerically bonded alkyl chains on silica L1
NUCLEOGEL®RP 300 polystyrene – divinylbenzene polymer L21
reversed phasechromatography of small molecules NUCLEOGEL®RP 100 small pore macroporous PS-DVB polymer L21
Food analysis – Sugars
RP chromatography of mono- and oligo-saccharides NUCLEOSIL®Carbohydrate silica-based special amino phase L8
separation of sugars, alcohols, organic acids based on ion exclusion, ion exchange, size exclusion, ligand exchange, NP and RP effects NUCLEOGEL® SUGAR 810 H PS-DVB resins with sulphonic acid modification in H form L17
NUCLEOGEL® SUGAR 810 Ca PS-DVB resins with sulphonic acid modification in Ca form L19
separation of sugars, alcohols, organic acids based on steric exclusion, ligand exchange and partition effects NUCLEOGEL®ION 300 OA PS-DVB resins with sulphonic acid modification in H form L17
NUCLEOGEL® SUGAR Ca PS-DVB resins with sulphonic acid modification in Ca form L19
NUCLEOGEL® SUGAR Pb PS-DVB resins with sulphonic acid modification in Pb form L34
NUCLEOGEL® SUGAR Na PS-DVB resins with sulphonic acid modification in Na form L58
Gel permeation chromatography (GPC)
water-insoluble compounds NUCLEOGEL®GPC polystyrene – divinylbenzene polymer

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