Sepax HPLC Columns

Reverse Phase
Normal Phase
Polar HILIC
Polymer Phase
UHPLC Phase

Reverse Phase

Phase Name	Silica	Modification	Particle Size		Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax GP-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 3, 4, 5, 7, and 10 µm	2.0-8.0	120 Å		17%	L1	300 m²/g
Sepax BR-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	1.5-10.5	120 Å	1.0ml/g	19%	L1	350 m²/g
Sepax HP-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped, Compatible with 100% aqueous mobile phase	1.8, 3, 4, 5, 7, and 10 µm	2.0-8.0	120 Å	1.0ml/g	17%	L1	300 m²/g
Sepax HP-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3 and 5 µm	2.0-8.0	200 Å	1.0ml/g	10	L1	200 m²/g
Sepax HP-C18(2)	Spherical, high purity (<10 ppm metals)	Special bonding density and proprietary endcapping	3 and 5 µm	2.0-8.0	100 Å	1.1mlg	17	L1	450 m²/g
Sepax Bio-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-8.0	200 Å	1.0ml/g	10	L1	200 m²/g
Sepax Bio-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-8.0	300 Å	0.95ml/g	7	L1	105 m²/g
Sepax GP-C8	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	11%	L7	300 m²/g
Sepax Bio-C8	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	300 Å	0.95ml/g	4%	L7	105 m²/g
Sepax GP-C4	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	8%	L26	300 m²/g
Sepax Bio-C4	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	300 Å	1.0ml/g	3%	L26	105 m²/g
Sepax GP-Phenyl	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5 µm	2.0-7.0	120 Å	1.0ml/g	11%	L11	300 m²/g

Normal Phase

Phase Name	Silica	Modification	Particle Size		Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax HP-Amino	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5 µm	2.0-7.0	120 Å	1.0ml/g	4%	L8	300 m²/g
Sepax HP-Cyano	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	7%	L10	300 m²/g
Sepax HP-Diol	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	80 & 120 Å	1.0ml/g	8.8%	L20	300 m²/g
Sepax HP-Silica	Sperical, high purity (<10 ppm metals)	activated hydroxyl (-OH)	1.8, 2.2, 3, 5, and 10 µm	2.0-7.0	60, 120, 200, 300, 500, 1000 and 2000 Å	1.0 ml/g for 120 Å pore size		L3	300 m²/g for 120 Å pore size

Mixed Mode Phase

Phase Name	Silica	Modification	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax HP-SCX	Spherical, high purity (<10 ppm metals)	Polymeric and mixed mode	1.8, 2.2, 3, 4, 5, 7, 10 µm	1.5-8.0	120 Å	1.0ml/g	11%	L9	300 m²/g
Sepax HP-SAX	Spherical, high purity (<10 ppm metals)	Polymeric and mixed mode	3, 4, 5, 7, 10 µm	1.5-8.0	120 Å	1.0ml/g	16%	L14	300 m²/g

Polymer Based Reverse Phase HPLC

Phase Name	PS/DVB Particles	Separation Mechanism	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
PolyRp	Spherical, 80% cross-linking	hydrophobic interaction	5, 10 µm	1.0-14.0	100,300,500,1000 Å	1.0ml/g	0%	L21	280 m²/g for 100A
PolyRp	Spherical, 80% cross-linking	hydrophobic interaction	1.7,3, 5, 10 µm	1.0-14.0	Non Porus	1.0ml/g	0%	L21	<10 m²/g

Polar HILIC Phase

Phase Name	Silica	Modification	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Polar Silica	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	0%		300 m²/g
Polar 100	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	16%		300 m²/g
Polar Diol	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8.8%		300 m²/g
Polar Pyridine	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8%		300 m²/g
Polar ImidazolE	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8.6%		300 m²/g

UHPLC Columns

Phases	Particle Size	Pore Size	Surface Area	Carbon Loading	pH Range
GP-C18	1.8, 2.2	120	300	17.0%	2-8.5
BR-C18	1.8, 2.2	120	300	19.0%	2-8.5
GP-C8	1.8, 2.2	120	300	11.0%	2-8.5
GP-C4	1.8, 2.2	120	300	8.0%	2-8.5
GP-Phenyl	1.8, 2.2	120	300	11.0%	2-8.5
HP-Amino	1.8, 2.2	120	300	7.0%	2-8.5
HP-Cyno	1.8, 2.2	120	300	4.0%	2-8.5
HP-SCX	1.8, 2.2	120	300	11.0%	2-8.5
HP-SAX	1.8, 2.2	120	300	16.0%	2-8.5
HP-Silica	1.8, 2.2	120	300	0.0%	2-8.5
Polar -100	1.8, 2.2	120	300	11.0%	2-8.5
PolyRP	1.7	Non-Porus			1-14

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